In this tutorial, we move from the basic biology of DNA, nucleosomes, promoters, enhancers, and transcription factors to the practical analysis of bulk ATAC-seq data. Using two human cell states—IMR90 differentiated fibroblast-like cells and GM23338 iPSC-like cells—we explore how chromatin accessibility differs between cellular states, from sequencing quality control and peak detection to consensus peaks, TSS annotation, motif enrichment, and nearest-gene mapping. The goal is not only to understand what ATAC-seq measures, but also to develop an intuitive understanding of how accessibility patterns reveal the regulatory architecture underlying cell identity.
This repository contains a comprehensive tutorial on Environmental Epidemiology. The document provides a structured introduction to the field, focusing on how environmental exposures influence population health and disease outcomes.
This post hosts a comprehensive document on Disability-Adjusted Life Years (DALY) and its role within the Global Burden of Disease (GBD) framework. The document provides a structured understanding of how population health is quantified by integrating both mortality and morbidity into a single metric.